TESTS FOR BILE SALTS AND ACETONE.
TESTS FOR BILE SALTS & ACETONE :-
■TESTS FOR BILE SALTS:-
a)PETTENKOFER'S TEST:-
ADD 5 DROPS OF 5% SUCROSE SOLUTION TO 5 ml OF TEST THE SOLUTION IN A TEST TUBE AND RUN A FEW ml OF CONCENTRATED H2SO4 CAREFULLY DOWN THE SIDE OF THE INCLINED TEST TUBE.
A PURPLE RING DEVELOPS SLOWLY.
b)HAY'S TEST(SURFACE TENSION TEST):-
SPRINKLE A SMALL AMOUNT OF FINELY POWDERED FLOWERS OF SULPHUR ON THE SURFACE OF 5 ml OF DISTILLED WATER AND 5 ml OF TEST SOLUTION TAKEN IN TWO SEPARATE TEST TUBES.
SULPHUR SINKS VERY RAPIDLY IN DILUTE BILE, BUT NOT IN WATER.
■TEST FOR ACETONE:-
a)LEGAL'S SODIUM NITROPRUSIDE TEST:-
ADD 3 DROPS OF A FRESHLY PREPARED 5% SODIUM NITROPRUSIDE SOLUTION TO ABOUT 3ml OF THE TEST SOLUTION,MIX AND MAKE ALKALINE WITH NaOH SOLUTION.
A RED COLOUR APPEARS.
ACIDIFY THE MIXTURE BY ADDING ACETIC ACID.
b)ROTHERA'S NITROPRISSIDE TEST:-
SATURATE 3 ml OF THE TEST SOLUTION (DILUTE ACETONE) WITH SOLID (NH4)2SO4 . ADD 2 DROPS OF A FRESHLY PREPARED 5% SODIUM NITROPRUSSIDE SOLUTION.FOUR CONCENTRATED AMMONIUM HYDROXIDE DOWN THE SIDE OF THE TEST TUBE.
A DEEP VIOLET COLOUR DEVELOPS AT THE SURFACE OF THE CONTACT.
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THANK TOU *LOVE YOU ALL 😍😚😘
■TESTS FOR BILE SALTS:-
a)PETTENKOFER'S TEST:-
ADD 5 DROPS OF 5% SUCROSE SOLUTION TO 5 ml OF TEST THE SOLUTION IN A TEST TUBE AND RUN A FEW ml OF CONCENTRATED H2SO4 CAREFULLY DOWN THE SIDE OF THE INCLINED TEST TUBE.
A PURPLE RING DEVELOPS SLOWLY.
b)HAY'S TEST(SURFACE TENSION TEST):-
SPRINKLE A SMALL AMOUNT OF FINELY POWDERED FLOWERS OF SULPHUR ON THE SURFACE OF 5 ml OF DISTILLED WATER AND 5 ml OF TEST SOLUTION TAKEN IN TWO SEPARATE TEST TUBES.
SULPHUR SINKS VERY RAPIDLY IN DILUTE BILE, BUT NOT IN WATER.
■TEST FOR ACETONE:-
a)LEGAL'S SODIUM NITROPRUSIDE TEST:-
ADD 3 DROPS OF A FRESHLY PREPARED 5% SODIUM NITROPRUSIDE SOLUTION TO ABOUT 3ml OF THE TEST SOLUTION,MIX AND MAKE ALKALINE WITH NaOH SOLUTION.
A RED COLOUR APPEARS.
ACIDIFY THE MIXTURE BY ADDING ACETIC ACID.
b)ROTHERA'S NITROPRISSIDE TEST:-
SATURATE 3 ml OF THE TEST SOLUTION (DILUTE ACETONE) WITH SOLID (NH4)2SO4 . ADD 2 DROPS OF A FRESHLY PREPARED 5% SODIUM NITROPRUSSIDE SOLUTION.FOUR CONCENTRATED AMMONIUM HYDROXIDE DOWN THE SIDE OF THE TEST TUBE.
A DEEP VIOLET COLOUR DEVELOPS AT THE SURFACE OF THE CONTACT.
_____________________________________________
THANK TOU *LOVE YOU ALL 😍😚😘
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